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Issue 1, 2005
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A microfluidic method for removal of the zona pellucida from mammalian embryos

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Abstract

Many in vitro procedures involve manipulation of the zona pellucida (chimerics, transgenics, biopsy). We have demonstrated a microfluidic channel network to precisely control (spatially and temporally) the delivery of chemical treatments for the removal of the zona pellucida. Building devices in polydimethylsiloxane (PDMS) with channel dimensions on the same order as that of the embryo diameter (∼120 µm) allows precise control of the local fluid environment. The system uses pressure driven flows to control embryo positioning, embryo movement, and plug formation. Zona removal is achieved by briefly washing a plug of lysing agent (acid Tyrode's medium) over the embryo.

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Publication details

The article was received on 04 May 2004, accepted on 15 Jul 2004 and first published on 14 Oct 2004


Article type: Technical Note
DOI: 10.1039/B406703G
Citation: Lab Chip, 2005,5, 108-110
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    A microfluidic method for removal of the zona pellucida from mammalian embryos

    H. C. Zeringue, M. B. Wheeler and D. J. Beebe, Lab Chip, 2005, 5, 108
    DOI: 10.1039/B406703G

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