Jump to main content
Jump to site search

Issue 6, 2004
Previous Article Next Article

Use of laminar flow patterning for miniaturised biochemical assays

Author affiliations

Abstract

Laminar flow in microfluidic chambers was used to construct low (one dimensional) density arrays suitable for miniaturized biochemical assays. By varying the ratio of flows of two guiding streams flanking a sample stream, precise focusing and positioning of the latter was achieved, and reactive species carried in the sample stream were deposited on functionalized chip surfaces as discrete 50 µm wide lanes. Using different model systems we have confirmed the method's suitability for qualitative screening and quantification tasks in receptor–ligand assays, recording biotin–streptavidin interactions, DNA-hybridization and DNA-triplex formation. The system is simple, fast, reproducible, flexible, and has small sample requirements.

Graphical abstract: Use of laminar flow patterning for miniaturised biochemical assays

Back to tab navigation

Publication details

The article was received on 16 Jun 2004, accepted on 24 Sep 2004 and first published on 11 Nov 2004


Article type: Paper
DOI: 10.1039/B409141H
Citation: Lab Chip, 2004,4, 654-657
  •   Request permissions

    Use of laminar flow patterning for miniaturised biochemical assays

    B. Regenberg, U. Krühne, M. Beyer, L. H. Pedersen, M. Simón, O. R. T. Thomas, J. Nielsen and T. Ahl, Lab Chip, 2004, 4, 654
    DOI: 10.1039/B409141H

Search articles by author

Spotlight

Advertisements