Issue 4, 2003

Nonlinear decrease of background fluorescence in polymer thin-films – a survey of materials and how they can complicate fluorescence detection in μTAS

Abstract

Polymers and plastics are receiving increased attention as materials for microfluidics and μTAS applications. Given the ubiquity of fluorescence detection techniques in micro-analytical systems, the fluorescence properties of polymers and plastics should not be overlooked. We survey some commonly available polymer thin-films for their fluorescence behaviour under standardized conditions to determine which materials are most suitable for high-sensitivity fluorescence detection lab chips. The initial fluorescence intensities of some of the materials surveyed were significantly higher than glass and fused silica controls, and decreased over the three hour period with complex kinetics. We then discuss how this has confounded fluorescence detection in our analytical context, and possible mechanisms for the decrease.

Article information

Article type
Paper
Submitted
08 Jul 2003
Accepted
28 Aug 2003
First published
18 Sep 2003

Lab Chip, 2003,3, 248-252

Nonlinear decrease of background fluorescence in polymer thin-films – a survey of materials and how they can complicate fluorescence detection in μTAS

K. R. Hawkins and P. Yager, Lab Chip, 2003, 3, 248 DOI: 10.1039/B307772C

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