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Issue 37, 2012
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A covalently modified hydrogel blend of hyaluronan–methyl cellulose with peptides and growth factors influences neural stem/progenitor cell fate

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Abstract

With the goal of controlling stem cell fate, for ultimate application in cell delivery, we synthesized a cell delivery vehicle comprised of an injectable hydrogel of hyaluronan (HA) and methyl cellulose (MC) wherein the MC is modified to allow bio-orthogonal coupling chemistry. Specifically, MC was modified using thiol-maleimide and biotin–streptavidin chemistry to covalently conjugate the cell adhesive peptide, glycine–arginine–glycine–aspartic acid–serine (GRGDS), and the oligodendrocyte-differentiating factor, recombinant platelet-derived growth factor A (rPDGF-A). NSPCs differentiated into significantly more oligodendrocytes when cultured in a HAMC hydrogel modified with both PDGF-A and GRGDS compared to controls of unmodified HAMC hydrogel and HAMC modified with only GRGDS. These results demonstrate both the ability to influence NSPC fate with immobilized growth factor and a platform technology for broad applicability in bioactive factor immobilization.

Graphical abstract: A covalently modified hydrogel blend of hyaluronan–methyl cellulose with peptides and growth factors influences neural stem/progenitor cell fate

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Publication details

The article was received on 07 Jun 2012, accepted on 12 Jul 2012 and first published on 13 Jul 2012


Article type: Paper
DOI: 10.1039/C2JM33680D
Citation: J. Mater. Chem., 2012,22, 19402-19411
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    A covalently modified hydrogel blend of hyaluronan–methyl cellulose with peptides and growth factors influences neural stem/progenitor cell fate

    R. Y. Tam, M. J. Cooke and M. S. Shoichet, J. Mater. Chem., 2012, 22, 19402
    DOI: 10.1039/C2JM33680D

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