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Issue 30, 2012
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Preparation of aminosilane–alginate hybrid microcapsules and their use for enzyme encapsulation

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Abstract

Organic–inorganic hybrid microcapsules (AP-capsules) were fabricated by sequential preparation of calcium alginate microcapsules (Alg-capsules), followed by the addition of the Alg-capsules to an aqueous solution of 3-aminopropyltriethoxysilane (APTES) in mild conditions without the use of any catalyst. Results showed that the shell thickness of AP-capsules was found to vary with time during the APTES incorporation onto the Alg-capsules, suggesting that the shell thickness was dependent on the amount of CaCl2 contained initially in the core solution as well as on the reaction time. A model enzyme, formate dehydrogenase (FDH), was successfully encapsulated in AP-capsules, and maximum efficiency of encapsulation was found with the capsule size of 1 mm diameter. The AP-capsules were reused efficiently for 10 cycles without loss of FDH activity. The approach developed in this study could evolve as a generic platform of enzyme immobilization for biotechnology applications using core–shell microcapsule technology.

Graphical abstract: Preparation of aminosilane–alginate hybrid microcapsules and their use for enzyme encapsulation

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Publication details

The article was received on 22 Mar 2012, accepted on 31 May 2012 and first published on 01 Jun 2012


Article type: Paper
DOI: 10.1039/C2JM31792C
Citation: J. Mater. Chem., 2012,22, 15405-15411
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    Preparation of aminosilane–alginate hybrid microcapsules and their use for enzyme encapsulation

    F. Kurayama, S. Suzuki, N. M. Bahadur, T. Furusawa, H. Ota, M. Sato and N. Suzuki, J. Mater. Chem., 2012, 22, 15405
    DOI: 10.1039/C2JM31792C

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