Issue 42, 2011

Direct enzyme patterning with microcontact printing and the growth of ZnO nanoparticles on the catalytic templates at room temperature

Abstract

Here we developed a microcontact printing (μCP) process to directly pattern enzymes in a single step without the loss of enzyme activity after printing. By modifying the substrate to display aldehyde groups, the direct stamping of urease enables the simultaneous patterning and covalent cross-linking of urease under the reducing agent NaCNBH4, which does not degrade the enzyme activity. Because the enzyme was not treated by the cross-linker prior to the sampling but rather pre-assembled on the surface of the substrate, only the contact areas of the PDMS stamp reacted with the cross-linker on the substrate, minimizing the poisoning of the enzymatic sites. The exposed urease particles on the substrate, free from the cross-linker, were still catalytically active and utilized to grow crystalline ZnO nanoparticles on the enzyme patterns in ambient conditions and in aqueous solution.

Graphical abstract: Direct enzyme patterning with microcontact printing and the growth of ZnO nanoparticles on the catalytic templates at room temperature

Additions and corrections

Article information

Article type
Communication
Submitted
15 Apr 2011
Accepted
07 Sep 2011
First published
29 Sep 2011

J. Mater. Chem., 2011,21, 16877-16879

Direct enzyme patterning with microcontact printing and the growth of ZnO nanoparticles on the catalytic templates at room temperature

K. I. Fabijanic, R. Perez-Castillejos and H. Matsui, J. Mater. Chem., 2011, 21, 16877 DOI: 10.1039/C1JM11609F

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