Issue 1, 2011

Assembly of an artificial biomembrane by encapsulation of an enzyme, formaldehyde dehydrogenase, into the nanoporous-walled silica nanotube–inorganic composite membrane

Abstract

We prepared silica nanotube with nanochannel-wall in the columnar pores of a commercial anodic alumina membrane (F127-MST), and then made up an artificial biomembrane consisting of a hierarchical structure by introducing an enzyme, formaldehyde dehydrogenase, into the silica nanochannels. The formaldehyde dehydrogenase confined in membrane showed a very high activity for the reduction of NAD+ similar to that of the native, besides much higher electron transfer to the electrode than the native. At the same time, we clarified that the encapsulation of enzymes into the nanochannels enables enzymes to accumulate in high density without aggregation and be arranged regularly. These results indicate that F127-MST can provide a favorable method for enzyme immobilization on the electrode.

Graphical abstract: Assembly of an artificial biomembrane by encapsulation of an enzyme, formaldehyde dehydrogenase, into the nanoporous-walled silica nanotube–inorganic composite membrane

Supplementary files

Article information

Article type
Paper
Submitted
20 May 2010
Accepted
07 Sep 2010
First published
20 Oct 2010

J. Mater. Chem., 2011,21, 251-256

Assembly of an artificial biomembrane by encapsulation of an enzyme, formaldehyde dehydrogenase, into the nanoporous-walled silica nanotube–inorganic composite membrane

T. Itoh, T. Shimomura, Y. Hasegawa, J. Mizuguchi, T. Hanaoka, A. Hayashi, A. Yamaguchi, N. Teramae, M. Ono and F. Mizukami, J. Mater. Chem., 2011, 21, 251 DOI: 10.1039/C0JM01523G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements