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Issue 39, 2010
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Superparamagnetic core-shell polymer particles for efficient purification of his-tagged proteins

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Abstract

Magnetic core-shell Fe3O4@SiO2@poly(styrene-alt-maleic anhydride) spheres enriched with Ni-NTA on their surface have been prepared by precipitation polymerization. The spheres have a core composed of superparamagnetic polycrystalline magnetite having a uniform size of ∼220 nm, endowing the spheres with excellent magnetic responsivity and dispersity. The shell composition of poly(styrene-alt-maleic anhydride) allows the incorporation of more Ni-NTA affinity sites onto the surface of the magnetic spheres. Owing to the multivalency effect, the separation capacity of His-tagged proteins by the as-prepared Fe3O4@SiO2@polymer/Ni-NTA composites was four times as that by Fe3O4@SiO2/Ni-NTA, making them particularly promising for the magnetic separation of low-concentration His-tagged proteins. The magnentic polymer hybrid particles also exhibited excellent performance in the direct separation of His-tagged proteins from cells lysates.

Graphical abstract: Superparamagnetic core-shell polymer particles for efficient purification of his-tagged proteins

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Publication details

The article was received on 01 Jul 2010, accepted on 12 Aug 2010 and first published on 07 Sep 2010


Article type: Paper
DOI: 10.1039/C0JM02081H
Citation: J. Mater. Chem., 2010,20, 8624-8630
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    Superparamagnetic core-shell polymer particles for efficient purification of his-tagged proteins

    W. Fang, X. Chen and N. Zheng, J. Mater. Chem., 2010, 20, 8624
    DOI: 10.1039/C0JM02081H

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