Issue 3, 2008

Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS

Abstract

Anion exchange-solid-phase extraction carried out prior to double column affinity-high performance liquid chromatography (AF-HPLC) coupled on-line with conventional inductively coupled plasma-(quadrupole) mass spectrometry is proposed for simultaneous speciation analysis of selenoprotein P (SelP), glutathione peroxidase (GPx) and selenoalbumin (SeAlb) in human serum. Efficient interference-free AF-HPLC separation of GPx, SelP and SeAlb is obtained within a total chromatographic run time less than 15 min. The repeatability in terms of relative standard deviation (R.S.D., %, n = 10) is 7% for GPx and SelP and 4% for SeAlb. The detection limits are 0.1 ng mL−1 for GPx, 1.0 ng mL−1 for SelP and 1.3 ng mL−1 for SeAlb. The method was validated by the analysis of two human serum reference materials certified for total Se content.

Graphical abstract: Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS

Article information

Article type
Technical Note
Submitted
21 Aug 2007
Accepted
20 Nov 2007
First published
30 Nov 2007

J. Anal. At. Spectrom., 2008,23, 402-406

Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS

P. Jitaru, M. Prete, G. Cozzi, C. Turetta, W. Cairns, R. Seraglia, P. Traldi, P. Cescon and C. Barbante, J. Anal. At. Spectrom., 2008, 23, 402 DOI: 10.1039/B712693J

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