Issue 9, 2000

Abstract

Chiral separation of underivatized selenoaminoacids enantiomers by high performance liquid chromatography (HPLC) on a glycopeptide antibiotic teicoplanin-based chiral stationary phase (Chirobiotic T) was investigated. The effect of methanol content, pH and ionic strength of the mobile phase on retention and resolution of the enantiomers was examined to optimize the chromatographic conditions. Underivatized DL-selenomethionine and DL-selenoethionine enantiomers were completely resolved within 8 min using a 2% (v/v) methanolwater (unbuffered) mobile phase delivered at 1 mL min−1. Very good selectivity and excellent detection limits of 1.9 µg L−1 (0.8 µg L−1 as Se) for each DL-selenomethionine enantiomer were obtaining by on-line coupling the chiral HPLC separation with selenium-specific detection by inductively coupled plasma quadrupole mass spectrometry (ICP-MS). The HPLC-ICP-MS method was successfully applied to the chiral speciation and determination of D- and L- selenomethionine enantiomers in real samples of selenized yeast.

Article information

Article type
Paper
Submitted
28 Feb 2000
Accepted
10 Jul 2000
First published
09 Aug 2000

J. Anal. At. Spectrom., 2000,15, 1109-1114

Chiral speciation and determination of selenomethionine enantiomers in selenized yeast by HPLC-ICP-MS using a teicoplanin-based chiral stationary phase

S. Pérez Méndez, E. Blanco González and A. Sanz Medel, J. Anal. At. Spectrom., 2000, 15, 1109 DOI: 10.1039/B001579M

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