Issue 9, 2004
From the journal:

Green Chemistry

Dissolution of Candida antarctica lipase B in ionic liquids: effects on structure and activity

Rute Madeira Lau,a   Menno J. Sorgedrager,a   Giacomo Carrea,b   Fred van Rantwijk,a   Francesco Secundob  and  Roger A. Sheldon*a  

* Corresponding authors

a Laboratory of Biocatalysis and Organic Chemistry, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands
E-mail: r.a.sheldon@tnw.tudelft.nl

b Istituto di Chimica del Riconoscimento Moleculare, CNR, Via Mario Bianco 9, Milano, Italy
E-mail: francesco.secundo@icrm.cnr.it

Abstract

The effects of ionic liquid media on the activity of Candida antarctica lipase B in a simple transesterification reaction were studied. In ionic liquids containing alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF4]. Only [Et3MeN][MeSO4] was an exception, as dissolved CaLB maintained its activity in this solvent. By means of FT-IR spectroscopy, denaturation of CaLB was observed upon dissolution in ionic liquids in which the activity was low, whereas the conformation of enzyme dissolved in [Et3MeN][MeSO4] closely resembled the native one.

Graphical abstract: Dissolution of Candida antarctica lipase B in ionic liquids: effects on structure and activity

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Article information

DOI
https://doi.org/10.1039/B405693K
Article type
Paper
Submitted
15 Apr 2004
Accepted
30 Jul 2004
First published
25 Aug 2004

Green Chem., 2004,6, 483-487

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Dissolution of Candida antarctica lipase B in ionic liquids: effects on structure and activity

R. Madeira Lau, M. J. Sorgedrager, G. Carrea, F. van Rantwijk, F. Secundo and R. A. Sheldon, Green Chem., 2004, 6, 483 DOI: 10.1039/B405693K

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