Functional food supplements to ameliorate the secondary complications in high fructose fed diabetic rats

Abstract

Functional foods are the most natural and safest source of health ingredients, providing health benefits beyond basic nutrition, and hence can be used as supplements for the prevention of secondary complications in diabetes. Persistent diabetes may cause glycation of various tissue proteins such as of those in lens, kidney, blood, and brain, which may further lead to the development of pathological conditions such as cataract and cardiovascular diseases. This study on adult rats was designed to assess if the functional food supplements A and B (proprietary blends of antioxidant rich plant materials) can reduce secondary complications such as cataract, dyslipidemia, and oxidative stress under severe diabetic conditions. After nine weeks of intervention of the supplements, it was found that the % HbA1c levels in the formulation group B significantly (p < 0.05) lowered (10.9%) followed by those in group A (11.1%) as compared to those in the diabetic fructose control (DFC) group (15.1%); moreover, plasma insulin levels were significantly (p < 0.01) improved in the formulation B group (9.8 mU L⁻¹) as compared to those in the DFC group (8.5 mU L⁻¹). The significantly higher level of plasma TEAC in group B (27.5 mg dL⁻¹) (p < 0.02) and group A (26.6 mg dL⁻¹) (p < 0.05) indicates an improved plasma antioxidants status as compared to that in DFC group (21.7 mg dL⁻¹). Both the formulation groups A and B showed a decrease in AGEs and tryptophan fluorescence, which suggests amelioration of the glycation of lens proteins as compared to that in the DFC group. The present results indicate that the formulations A and B exhibit antiglycating and antioxidant potentials by inhibiting the high fructose-induced glycation in diabetic rats; hence, they may have therapeutic value as functional foods in the effective management of secondary complications associated with severe diabetic conditions.