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Issue 7, 2015
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The anti-infective activity of punicalagin against Salmonella enterica subsp. enterica serovar typhimurium in mice

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Abstract

Punicalagin, a major bioactive component of pomegranate peel, has been proven to have antioxidant, antiviral, anti-apoptosis, and hepatoprotective properties. The aim of this study was to investigate the anti-infective activity of punicalagin in a mouse model. C57BL/6 mice were initially challenged with Salmonella enterica subsp. enterica serovar typhimurium (S. typhimurium) and then treated with punicalagin. Food and water consumption and body weight were recorded daily. On day 8 post infection, the mice were sacrificed to examine pathogen counts in tissues, hematological parameters, cytokine levels, and histological changes. Compared to mice only infected with S. typhimurium, punicalagin-treated mice had more food consumption and less weight loss. A higher survival rate and lower counts of viable S. typhimurium in feces, liver, spleen, and kidney were found in the punicalagin-treated mice. The enzyme linked immunosorbent assay showed that the levels of IL-6, IL-10, and IFN-γ in serum and the spleen and TNF-α in serum, the spleen and the liver were reduced by punicalagin. Moreover, more neutrophils and higher neutrophil-to-mononuclear cell ratios in the punicalagin-treated mice were observed. Histological examination showed that punicalagin protected cells in the liver and spleen from hemorrhagic necrosis. It is concluded that punicalagin has a beneficial effect against S. typhimurium infection in mice. The anti-infective properties, together with other nutritionally beneficial effects, make punicalagin a promising supplement in human food or animal feeds to prevent disease associated with S. typhimurium.

Graphical abstract: The anti-infective activity of punicalagin against Salmonella enterica subsp. enterica serovar typhimurium in mice

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Publication details

The article was received on 14 Jan 2015, accepted on 31 May 2015 and first published on 01 Jun 2015


Article type: Paper
DOI: 10.1039/C5FO00053J
Author version available: Download Author version (PDF)
Citation: Food Funct., 2015,6, 2357-2364
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    The anti-infective activity of punicalagin against Salmonella enterica subsp. enterica serovar typhimurium in mice

    G. Li, Y. Feng, Y. Xu, Q. Wu, Q. Han, X. Liang, B. Yang, X. Wang and X. Xia, Food Funct., 2015, 6, 2357
    DOI: 10.1039/C5FO00053J

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