Issue 3, 2013

Antioxidant activity and oxidative stress protection of duck proteins hydrolysates in SK-N-SH cells

Abstract

Studies have found that natural antioxidants, which are free-radical scavengers, can reduce the risk of diseases caused by free radicals. This work investigated the antioxidant properties of duck proteins hydrolysates. The free-radical scavenging function of CP-1 (Mr > 10 kDa), CP-2 (5 kDa < Mr < 10 kDa) and CP-3 (Mr < 5 kDa), obtained through ultrafiltration and gel filtration were evaluated. The results showed that the lower molecular weight fraction exhibited a stronger free-radical scavenging ability. The highest free-radical scavenging activity was detected in the fraction of p4 purified from CP-3 using Sephadex G-15 column chromatography. The 50% inhibitory value (IC50) of p4 for scavenging radicals of superoxide, hydroxyl and 1,1-diphenyl-2-pycrylhydrazyl (DPPH) were, respectively, 0.97 mg mL−1, 0.84 mg mL−1 and 1.84 mg mL−1. Furthermore, the p4 fraction at a concentration of 10 μg mL−1 increased cell viability from 84.8% to 94% under antioxidative stress in neuroblastoma SK-N-SH cells.

Graphical abstract: Antioxidant activity and oxidative stress protection of duck proteins hydrolysates in SK-N-SH cells

Article information

Article type
Paper
Submitted
27 Sep 2012
Accepted
12 Dec 2012
First published
14 Dec 2012

Food Funct., 2013,4, 476-480

Antioxidant activity and oxidative stress protection of duck proteins hydrolysates in SK-N-SH cells

Y. Guo, D. Pan, Z. Wu, C. Zhao and J. Cao, Food Funct., 2013, 4, 476 DOI: 10.1039/C2FO30264K

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