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Issue 8, 2012
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Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

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Abstract

This study assesses the efficacy of anthocyanin rich Brassica olerecea leaves (ARCE) and flavonoid rich Eugenia jambolana seed (EJSE) extracts as possible cardioprotective agents against hydrogen peroxide (H2O2) induced cytotoxicity in H9C2 cells. Presence of ARCE or EJSE resulted in a superior cell viability and cell integrity as revealed by cell viability and lactate dehydrogenase release assays and acridine orange and ethidium bromide staining of control and H2O2 treated H9C2 cells. These extracts were also able to reduce the impact of H2O2 induced lipid peroxidation and depletion of intracellular glutathione. Also, there was an increase in mitochondrial membrane potential and reduced generation of intracellular reactive oxygen species following ARCE or EJSE treatments. These results suggest that ARCE and EJSE are capable of cardioprotective activity due to the high number of anthocyanins and flavonoids in them that are instrumental in lowering intracellular oxidative stress, preventing depletion of cellular antioxidants and improving cell viability.

Graphical abstract: Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

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Publication details

The article was received on 01 Jan 2012, accepted on 18 Apr 2012 and first published on 17 May 2012


Article type: Paper
DOI: 10.1039/C2FO00001F
Citation: Food Funct., 2012,3, 837-843
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    Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

    R. V. Devkar, A. V. Pandya and N. H. Shah, Food Funct., 2012, 3, 837
    DOI: 10.1039/C2FO00001F

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