Issue 3, 2017

Poly(acrylic acid)-coated titanium dioxide nanoparticle and ultraviolet light co-exposure has minimal effect on developing zebrafish (Danio rerio)

Abstract

Widespread production of titanium dioxide nanoparticles (TiO2 NPs) for use in various consumer products has increased the potential for their release into the environment. Most studies examine TiO2 NP toxicity under laboratory lighting that emits little to no ultraviolet (UV) radiation, yet these nanoscale particles are wide band-gap semiconductors capable of generating reactive oxygen species. The purpose of this study was to examine poly(acrylic acid)-coated TiO2 NP phototoxicity in vivo. Zebrafish (Danio rerio) embryos were exposed to 0.1, 1 or 10 mg L−1 of characterized uncoated TiO2 NPs (positive control), polymer-coated TiO2 NPs, and the polymer coating alone (negative control) in the presence or absence of UV light. Survival, hatching success, malformation, thiobarbituric acid reactive substances (TBARS), catalase activity, total glutathione (TG) level, superoxide dismutase activity, and alteration in antioxidant-related gene expression were measured. Uncoated TiO2 NPs produced hydroxyl radicals (OH˙), delayed hatch, induced lipid peroxidation, increased catalase activity and TG levels, and upregulated glutathione peroxidase 1a gene expression 1.59 fold in the presence of UV light. Polymer-coated TiO2 NP increased both TBARS production and TG levels under simulated sunlight illumination. We suggest that the polymer coating plays a role in OH˙-mediated effects of TiO2 NPs in developing zebrafish and that toxicity depends on UV illumination. Since the presence of UV light evidently affects TiO2 NP toxicity, the need for more environmentally representative nanotoxicity studies that take sunlight activation into account is strengthened.

Graphical abstract: Poly(acrylic acid)-coated titanium dioxide nanoparticle and ultraviolet light co-exposure has minimal effect on developing zebrafish (Danio rerio)

Supplementary files

Article information

Article type
Paper
Submitted
26 Sep 2016
Accepted
27 Jan 2017
First published
30 Jan 2017

Environ. Sci.: Nano, 2017,4, 658-669

Poly(acrylic acid)-coated titanium dioxide nanoparticle and ultraviolet light co-exposure has minimal effect on developing zebrafish (Danio rerio)

L. C. Felix, E. J. Folkerts, Y. He and G. G. Goss, Environ. Sci.: Nano, 2017, 4, 658 DOI: 10.1039/C6EN00436A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements