Jump to main content
Jump to site search

Issue 6, 2005
Previous Article Next Article

Adenine nucleotides in snail muscles as one of biomarkers of fluoride toxicity

Author affiliations

Abstract

The aim of this work was to determine the extent of bioaccumulation of fluorides in tissues of Helix aspersa maxima. The toxicity of fluorides administered orally on the energy balance of the snail’s foot was investigated based on measurements of concentrations of adenine nucleotides and their metabolism degradation products. Quantitation of fluoride levels was done in soft tissues (foot, hepatopancreas) and shells of mature snails. Qualitative and quantitative analysis of purine compounds was performed in slices of foot from mature snails. Fluoride concentrations in pulverized shells were measured using an ion-selective electrode. Gas chromatography was used to determine fluoride concentrations in soft tissues (hepatopancreas and foot). Purines were measured in foot muscle slices with high-performance liquid chromatography (HPLC). Fluoride levels in soft tissues of the snail cannot serve as an indicator for biomonitoring purposes as no significant accumulation was observed during exposure to maximum allowable concentrations of fluoride in drinking water. Contrary to this, levels of fluoride in the shell rose significantly with this concentration of fluoride in drinking water. The effect of fluorides on energy metabolism of foot muscle was evidenced by elevated AMP levels, increased adenine nucleotide pool and reduced conversion of ADP to ATP. Exposure to rising F concentrations was accompanied by decreasing values of the adenylate energy charge AEC. Determination of AMP or AEC in foot muscle of exposed snails seems to be a useful indicator of fluoride effects on metabolic activity.

Graphical abstract: Adenine nucleotides in snail muscles as one of biomarkers of fluoride toxicity

Back to tab navigation

Publication details

The article was received on 12 Oct 2004, accepted on 04 Mar 2005 and first published on 16 May 2005


Article type: Paper
DOI: 10.1039/B415833B
Citation: J. Environ. Monit., 2005,7, 631-634
  •   Request permissions

    Adenine nucleotides in snail muscles as one of biomarkers of fluoride toxicity

    M. Rac, K. Safranow, K. Jakubowska, D. Chlubek and Z. Machoy, J. Environ. Monit., 2005, 7, 631
    DOI: 10.1039/B415833B

Search articles by author

Spotlight

Advertisements