Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms

A. D'Urso; R. Randazzo; V. Rizzo; C. M. A. Gangemi; V. Romanucci; A. Zarrelli; G. Tomaselli; D. Milardi; N. Borbone; R. Purrello; G. Piccialli; G. Di Fabio; G. Oliviero

doi:10.1039/C7CP02816D

Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms†

A. D'Urso,

*^a R. Randazzo,^a V. Rizzo,^a C. M. A. Gangemi,^a V. Romanucci,^b A. Zarrelli,^b G. Tomaselli,

^a D. Milardi,^c N. Borbone,

^d R. Purrello,

^a G. Piccialli,^de G. Di Fabio^b and G. Oliviero*^f

Author affiliations

* Corresponding authors

^a Department of Chemical Science, University of Catania, V.le A Doria 6, Catania, Italy
E-mail: adurso@unict.it

^b Department of Chemical Science, University of Naples Federico II, Monte Sant’Angelo Via Cintia, Napol, Italy

^c Institute of Biostructures and Bioimaging, IBB-CNR UOS of Catania, Via P. Gaifami 18 95126, Catania, Italy

^d Department of Pharmacy, University of Naples Federico II, D. Montesano 49, 80131 Napoli, Italy

^e Institute of protein Biochemistry, IPB-CNR, Via Pietro Castellino 111, 80131 Napoli, Italy

^f Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Napoli, Italy
E-mail: golivier@unina.it

Abstract

The interaction of the porphyrin derivative H₂TCPPSpm4, having spermine pendants in the four meso positions, with the G-quadruplex (GQ) structure formed by the DNA aptamer TGGGAG has been investigated by means of UV, electronic circular dichroism and PAGE studies. The results reported here demonstrate that the porphyrin derivative is capable of stabilizing or destabilizing the higher-ordered structures of parallel GQs, depending on the method used to reach their relative stoichiometry (titration vs. single addition). Noteworthily, when two equivalents of H₂TCPPSpm4 were mixed directly with one equivalent of the (TGGGAG)₄ GQ to reach a 2 : 1 H₂TCPPSpm4 : GQ ratio T_1/2 higher than 80 °C was also observed confirming the presence of higher-ordered GQ structures.

Physical Chemistry Chemical Physics

Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms†

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Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms

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