Issue 2, 2013
From the journal:

Physical Chemistry Chemical Physics

Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptideinhibitor siamycin I revealed through synchrotron radiation circular dichroism†

Mary K. Phillips-Jones,‡*a   Simon G. Patching,‡bc   Shalini Edara,b   Jiro Nakayama,d   Rohanah Hussaine  and  Giuliano Siligardi*e  

* Corresponding authors

a School of Pharmacy and Biomedical Sciences, Faculty of Science and Technology, University of Central Lancashire, Preston, Lancashire, UK
E-mail: MPhillips-Jones@uclan.ac.uk
Fax: +44 (0)1772 892929
Tel: +44 (0)1772 895831

b Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK
E-mail: S.G.Patching@leeds.ac.uk, eshalini@hotmail.co.uk
Fax: +44 (0)113 343 3069
Tel: +44 (0)113 343 3172

c School of Biomedical Sciences, University of Leeds, Leeds, UK

d Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan
E-mail: nakayama@agr.kyushu-u.ac.jp

e Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, UK
E-mail: rohanah.hussain@diamond.ac.uk, giuliano.siligardi@diamond.ac.uk

Abstract

The suitability of synchrotron radiation circular dichroism spectroscopy (SRCD) for studying interactions between the tricyclic peptide inhibitor siamycin I and the intact FsrC membrane sensor kinase in detergent micelles has been established. In the present study, tertiary structural changes demonstrate that inhibitor binding occurs at a different, non-overlapping site to the native ligand, GBAP.

Graphical abstract: Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism

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Article information

DOI
https://doi.org/10.1039/C2CP43722H
Article type
Communication
Submitted
22 Oct 2012
Accepted
13 Nov 2012
First published
14 Nov 2012

Phys. Chem. Chem. Phys., 2013,15, 444-447

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Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism

M. K. Phillips-Jones, S. G. Patching, S. Edara, J. Nakayama, R. Hussain and G. Siligardi, Phys. Chem. Chem. Phys., 2013, 15, 444 DOI: 10.1039/C2CP43722H

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