Issue 12, 2013

Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Abstract

Hyperspectral microscopy is a versatile method for simultaneous spatial and spectroscopic characterization of nonfluorescent samples. Here we present a hyperspectral darkfield imaging system for spectral imaging of single nanoparticles over an area of 150 × 150 μm2 and at illumination intensities compatible with live cell imaging. The capabilities of the system are demonstrated using correlated transmission electron microscopy and single-particle optical studies of colloidal hollow gold nanoparticles. The potential of the system for characterizing the interactions between nanoparticles and cells has also been demonstrated. In this case, the spectral information proves a useful improvement to standard darkfield imaging as it enables differentiation between light scattered from nanoparticles and light scattered from other sources in the cellular environment. The combination of low illumination power and fast integration times makes the system highly suitable for nanoparticle tracking and spectroscopy in live-cell experiments.

Graphical abstract: Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Article information

Article type
Paper
Submitted
09 Sep 2012
Accepted
02 Nov 2012
First published
08 Nov 2012

Phys. Chem. Chem. Phys., 2013,15, 4163-4168

Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

N. Fairbairn, A. Christofidou, A. G. Kanaras, T. A. Newman and O. L. Muskens, Phys. Chem. Chem. Phys., 2013, 15, 4163 DOI: 10.1039/C2CP43162A

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