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PELDOR study on the tyrosyl radicals in the R2 protein of mouse ribonucleotide reductase†
Abstract
Pulse electron–electron double resonance (
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Supplementary files
- Fig. 3 The three- and four-pulse PELDOR sequences used in this work.The detection (D) and pumping (P) pulse lengths employed for the PELDOR experiments are listed. X-band 3-pulse: (D) 16 ns?32 ns, (P) 32 ns. X-band four-pulse: (D) 16 ns?32 ns?32 ns, (P) 32 ns. Q-band three-pulse: (D) 148 ns?300 ns, (P) 60 ns. TIF (627K)
- Fig. 4 (A) Echo detected X-band EPR spectrum of mouse-R2-d; the arrows indicate the frequencies of the detection (D) and pumping (P) pulses; (B) Four-pulse X-band PELDOR of mouse-R2-d: experimental (black) and simulated (red) spectrum computed with the DeerAnalysis 2004 program. (C) Analysis of distance distribution of the two Y radicals in the dimer obtained with the DeerAnalysis 2004 program. For further experimental conditions see both Fig. 3 and Fig. 5. TIF (136K)
Article information
- Article type
- Communication
- Submitted
- 03 Oct 2005
- Accepted
- 10 Nov 2005
- First published
- 21 Nov 2005
Phys. Chem. Chem. Phys., 2006,8, 58-62
Permissions
PELDOR study on the tyrosyl radicals in the R2 protein of mouse ribonucleotide reductase
D. Biglino, P. P. Schmidt, E. J. Reijerse and W. Lubitz, Phys. Chem. Chem. Phys., 2006, 8, 58 DOI: 10.1039/B513950C
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