Issue 46, 2018
From the journal:

Chemical Communications

Integration of single-molecule detection with magnetic separation for multiplexed detection of DNA glycosylases†

Chen-chen Li,‡a   Yan Zhang,‡a   Bo Tang ORCID logo *a  and  Chun-yang Zhang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn, tangb@sdnu.edu.cn
Fax: +86-531-82615258
Tel: +86-531-86186033

Abstract

We combine single-molecule detection with magnetic separation for simultaneous measurement of human 8-oxoG DNA glycosylase 1 (hOGG1) and uracil DNA glycosylase (UDG) based on excision repair-initiated endonuclease IV (Endo IV)-assisted signal amplification. This method can sensitively detect multiple DNA glycosylases, and it can be further applied for the simultaneous measurement of enzyme kinetic parameters and screening of both hOGG1 and UDG inhibitors.

Graphical abstract: Integration of single-molecule detection with magnetic separation for multiplexed detection of DNA glycosylases

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Article information

DOI
https://doi.org/10.1039/C8CC01695J
Article type
Communication
Submitted
01 Mar 2018
Accepted
18 Apr 2018
First published
20 Apr 2018

Chem. Commun., 2018,54, 5839-5842

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Integration of single-molecule detection with magnetic separation for multiplexed detection of DNA glycosylases

C. Li, Y. Zhang, B. Tang and C. Zhang, Chem. Commun., 2018, 54, 5839 DOI: 10.1039/C8CC01695J

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