Issue 96, 2017

Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

Abstract

We showcase the high potential of the 2′-cyanoethoxymethyl (CEM) methodology to synthesize RNAs with naturally occurring modified residues carrying stable isotope (SI) labels for NMR spectroscopic applications. The method was applied to synthesize RNAs with sizes ranging between 60 to 80 nucleotides. The presented approach gives the possibility to selectively modify larger RNAs (>60 nucleotides) with atom-specifically 13C/15N-labelled building blocks. The method harbors the unique potential to address structural as well as dynamic features of these RNAs with NMR spectroscopy but also using other biophysical methods, such as mass spectrometry (MS), or small angle neutron/X-ray scattering (SANS, SAXS).

Graphical abstract: Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

Supplementary files

Article information

Article type
Communication
Submitted
29 Aug 2017
Accepted
28 Sep 2017
First published
20 Nov 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 12938-12941

Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

J. Kremser, E. Strebitzer, R. Plangger, M. A. Juen, F. Nußbaumer, H. Glasner, K. Breuker and C. Kreutz, Chem. Commun., 2017, 53, 12938 DOI: 10.1039/C7CC06747J

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