Issue 76, 2017

Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching

Abstract

Herein, we develop a simple fluorescence method for the sensitive detection of microRNAs based on duplex specific nuclease-assisted target recycling and pyrene excimer switching. This method is extremely sensitive with a detection limit of 0.58 fM, and it can be applied to absolutely detect endogenous microRNAs in cancer cells, holding great potential for clinical diagnosis and biomedical research studies.

Graphical abstract: Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching

Supplementary files

Article information

Article type
Communication
Submitted
11 Aug 2017
Accepted
05 Sep 2017
First published
05 Sep 2017

Chem. Commun., 2017,53, 10596-10599

Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching

F. Ma, W. Liu, Q. Zhang and C. Zhang, Chem. Commun., 2017, 53, 10596 DOI: 10.1039/C7CC06290G

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