Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging

Abstract

A facile method for the preparation of antibody–quantum dot (QD) conjugates using the immunoglobulin binding (B1) domain of protein G is presented. The utility of antibody–QD conjugates using the B1 domain is demonstrated for fluorescence imaging of breast tumor cells in vitro and in vivo.