Issue 54, 2017

An efficient target–intermediate recycling amplification strategy for ultrasensitive fluorescence assay of intracellular lead ions

Abstract

An ultrasensitive fluorescence assay for intracellular Pb2+ determination was proposed through target–intermediate recycling amplification based on metal-assisted DNAzyme catalysis and strand displacement reactions. Compared with only target recycling-based fluorescence assay with an M amplification ratio, the proposed assay could achieve an M × N amplification ratio to obtain an improved sensitivity by more than 10 times, in which M and N are the amplification ratios of target recycling and intermediate recycling, respectively. Remarkably, this proposed ultrasensitive fluorescence assay could be applied to the determination of various analytes with the well-designed detection probe, especially in intracellular assay, providing a promising tool for clinical diagnosis and biomedical detection.

Graphical abstract: An efficient target–intermediate recycling amplification strategy for ultrasensitive fluorescence assay of intracellular lead ions

Supplementary files

Article information

Article type
Communication
Submitted
27 May 2017
Accepted
13 Jun 2017
First published
13 Jun 2017

Chem. Commun., 2017,53, 7525-7528

An efficient target–intermediate recycling amplification strategy for ultrasensitive fluorescence assay of intracellular lead ions

Z. Wen, W. Liang, Y. Zhuo, C. Xiong, Y. Zheng, R. Yuan and Y. Chai, Chem. Commun., 2017, 53, 7525 DOI: 10.1039/C7CC04104G

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