Issue 51, 2017

The efficient synthesis and purification of amyloid-β(1–42) using an oligoethylene glycol-containing photocleavable lysine tag

Abstract

A short, monodisperse oligoethylene glycol-containing photocleavable lysine tag was developed to facilitate the efficient purification of hydrophobic and fibril-forming peptides. This new tag was used to prepare a modified Aβ42 peptide with increased solubility and decreased propensity to aggregate in aqueous media. The solubilising tag was readily removed by irradiation with UV light and permitted the preparation and isolation of Aβ42 in high purity and yield.

Graphical abstract: The efficient synthesis and purification of amyloid-β(1–42) using an oligoethylene glycol-containing photocleavable lysine tag

Supplementary files

Article information

Article type
Communication
Submitted
24 Apr 2017
Accepted
06 Jun 2017
First published
06 Jun 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 6903-6905

The efficient synthesis and purification of amyloid-β(1–42) using an oligoethylene glycol-containing photocleavable lysine tag

J. A. Karas, A. Noor, C. Schieber, T. U. Connell, F. Separovic and P. S. Donnelly, Chem. Commun., 2017, 53, 6903 DOI: 10.1039/C7CC03147E

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