Fluorescence turn-on hairpin-probe PCR

Abstract

A new fluorescence turn-on type of PCR monitoring system (Hpro-PCR) using a hairpin probe and a primer having a tag sequence at the 5′ end with the fluorescent molecule 2,7-diamino-1,8-naphthyridine derivative (DANP) has been developed. The Hpro-PCR exploited the modulation of the equilibrium states between the DANP-bound hairpin structure and probe–tag duplex, and the PCR progress alternated the equilibrium state, resulting in the change of fluorescent intensity.