A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging

F. Doria; M. Nadai; M. Zuffo; R. Perrone; M. Freccero; S. N. Richter

doi:10.1039/C6CC08492C

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A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging†

F. Doria,

‡^a M. Nadai,

‡^b M. Zuffo,^a R. Perrone,

^b M. Freccero

*^a and S. N. Richter*^b

Author affiliations

* Corresponding authors

^a Dept. of Chemistry, University of Pavia, V.le Taramelli 10, 27100 Pavia, Italy
E-mail: mauro.freccero@unipv.it

^b Dept. of Molecular Medicine, University of Padua, via Gabelli 63, 35121 Padua, Italy
E-mail: sara.richter@unipd.it

Abstract

Aggregation, red-NIR emission and light-up upon nucleic acid G-quadruplex binding have been investigated for a prototype core-extended naphthalene diimide, which is capable of fast cellular entry and nucleolar localization. Both high-level colocalization with an anti-G-quadruplex antibody and nucleolin displacement reveal that the compound targets and thus makes visible nuclear DNA G-quadruplexes.

This article is part of the themed collection: Suprabiomolecular Chemistry

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Article information

DOI: https://doi.org/10.1039/C6CC08492C
Article type: Communication
Submitted: 21 Oct 2016
Accepted: 23 Jan 2017
First published: 23 Jan 2017
This article is Open Access

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Chem. Commun., 2017,53, 2268-2271

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A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging

F. Doria, M. Nadai, M. Zuffo, R. Perrone, M. Freccero and S. N. Richter, Chem. Commun., 2017, 53, 2268 DOI: 10.1039/C6CC08492C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Chemical Communications

A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging†

Abstract

Supplementary files

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A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging

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