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Issue 66, 2016
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Rapid protein immobilization for thin film continuous flow biocatalysis

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Abstract

A versatile enzyme immobilization strategy for thin film continuous flow processing is reported. Here, non-covalent and glutaraldehyde bioconjugation are used to immobilize enzymes on the surfaces of borosilicate reactors. This approach requires only ng of protein per reactor tube, with the stock protein solution readily recycled to sequentially coat >10 reactors. Confining reagents to thin films during immobilization reduced the amount of protein, piranha-cleaning solution, and other reagents by ∼96%. Through this technique, there was no loss of catalytic activity over 10 h processing. The results reported here combines the benefits of thin film flow processing with the mild conditions of biocatalysis.

Graphical abstract: Rapid protein immobilization for thin film continuous flow biocatalysis

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Publication details

The article was received on 19 May 2016, accepted on 18 Jul 2016 and first published on 19 Jul 2016


Article type: Communication
DOI: 10.1039/C6CC04210D
Citation: Chem. Commun., 2016,52, 10159-10162
  • Open access: Creative Commons BY license
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    Rapid protein immobilization for thin film continuous flow biocatalysis

    J. Britton, C. L. Raston and G. A. Weiss, Chem. Commun., 2016, 52, 10159
    DOI: 10.1039/C6CC04210D

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