Issue 36, 2016

A FRET-enabled molecular peptide beacon with a significant red shift for the ratiometric detection of nucleic acids

Abstract

A cationic molecular peptide beacon NAP1 functionalized with a fluorescence resonance energy transfer-pair at its ends allows the ratiometric detection of ds-DNA with a preference for AT rich sequences. NAP1 most likely binds in a folded form into the minor groove of ds-DNA, which results in a remarkable change in its fluorescence properties. As NAP1 exhibits quite low cytotoxicity, it can also be used for imaging of nuclear DNA in cells.

Graphical abstract: A FRET-enabled molecular peptide beacon with a significant red shift for the ratiometric detection of nucleic acids

Supplementary files

Article information

Article type
Communication
Submitted
10 Mar 2016
Accepted
04 Apr 2016
First published
04 Apr 2016

Chem. Commun., 2016,52, 6134-6137

A FRET-enabled molecular peptide beacon with a significant red shift for the ratiometric detection of nucleic acids

D. Maity, J. Jiang, M. Ehlers, J. Wu and C. Schmuck, Chem. Commun., 2016, 52, 6134 DOI: 10.1039/C6CC02138G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements