Issue 24, 2016
From the journal:

Chemical Communications

Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes†

Hideki Itoh,ab   Satoshi Arai,cd   Thankiah Sudhaharan,b   Sung-Chan Lee,e   Young-Tae Chang,fg   Shin'ichi Ishiwata,*cdh   Madoka Suzuki*cd  and  E. Birgitte Lane*b  

* Corresponding authors

a Department of Pure and Applied Physics, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 169-8555, Japan

b Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore
E-mail: birgit.lane@imb.a-star.edu.sg

c Organization for University Research Initiatives, Waseda University, Tokyo 162-0041, Japan
E-mail: suzu_mado@aoni.waseda.jp, Ishiwata@waseda.jp

d Waseda Bioscience Research Institute in Singapore (WABIOS), Singapore 138667, Singapore

e Aptabio Therapeutics Inc., Yongin 446-908, Korea

f Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 138667, Singapore

g Department of Chemistry and MedChem Program of Life Sciences Institute, National University of Singapore, Singapore 117543, Singapore

h Department of Physics, Faculty of Advanced Science and Engineering, Waseda University, Tokyo 169-8555, Japan

Abstract

We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.

Graphical abstract: Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

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Article information

DOI
https://doi.org/10.1039/C5CC09943A
Article type
Communication
Submitted
03 Dec 2015
Accepted
21 Jan 2016
First published
21 Jan 2016
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2016,52, 4458-4461

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Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

H. Itoh, S. Arai, T. Sudhaharan, S. Lee, Y. Chang, S. Ishiwata, M. Suzuki and E. B. Lane, Chem. Commun., 2016, 52, 4458 DOI: 10.1039/C5CC09943A

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