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Issue 4, 2016
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The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

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Abstract

We developed a novel strategy by oxidation–derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.

Graphical abstract: The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

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The article was received on 02 Sep 2015, accepted on 03 Nov 2015 and first published on 03 Nov 2015


Article type: Communication
DOI: 10.1039/C5CC07354E
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Citation: Chem. Commun., 2016,52, 737-740
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    The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

    H. Zhang, J. Xiong, B. Qi, Y. Feng and B. Yuan, Chem. Commun., 2016, 52, 737
    DOI: 10.1039/C5CC07354E

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