Click chemistry-mediated catalytic hairpin self-assembly for amplified and sensitive fluorescence detection of Cu2+ in human serum

Daxiu Li; Wenjiao Zhou; Yaqin Chai; Ruo Yuan; Yun Xiang

doi:10.1039/C5CC04218F

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Click chemistry-mediated catalytic hairpin self-assembly for amplified and sensitive fluorescence detection of Cu²⁺ in human serum†

Daxiu Li,^a Wenjiao Zhou,^a Yaqin Chai,^a Ruo Yuan^a and Yun Xiang*^a

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* Corresponding authors

^a Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
E-mail: yunatswu@swu.edu.cn
Fax: +86-23-68252277
Tel: +86-23-68253172

Abstract

Chemically reduced Cu²⁺ triggers the ligation of alkynyl- and azido-modified DNA via click chemistry. Subsequently, the ligated DNA initiates cyclic assembly of two fluorescently quenched hairpin DNAs and generates significantly amplified fluorescence signals for highly sensitive detection of Cu²⁺ in human serum samples.

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Article information

DOI: https://doi.org/10.1039/C5CC04218F
Article type: Communication
Submitted: 21 May 2015
Accepted: 01 Jul 2015
First published: 01 Jul 2015

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Chem. Commun., 2015,51, 12637-12640

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Click chemistry-mediated catalytic hairpin self-assembly for amplified and sensitive fluorescence detection of Cu²⁺ in human serum

D. Li, W. Zhou, Y. Chai, R. Yuan and Y. Xiang, Chem. Commun., 2015, 51, 12637 DOI: 10.1039/C5CC04218F

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Chemical Communications