Issue 19, 2015

Oligonucleotides containing a piperazino-modified 2′-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance

Abstract

Incorporation of a piperazino-modified 2′-amino-LNA monomer (PipLNA-T) into oligonucleotides conferred very high affinity and base-pairing selectivity towards complementary DNA and RNA strands. Furthermore, one PipLNA-T modification provided a robust nuclease resistance that safeguarded three neighbouring natural nucleosides from 3′-exonucleolytic degradation. These favourable properties render PipLNA-T a promising oligonucleotide modification for various biological applications.

Graphical abstract: Oligonucleotides containing a piperazino-modified 2′-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance

Supplementary files

Article information

Article type
Communication
Submitted
13 Jan 2015
Accepted
03 Feb 2015
First published
03 Feb 2015
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 4024-4027

Oligonucleotides containing a piperazino-modified 2′-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance

C. Lou, B. Vester and J. Wengel, Chem. Commun., 2015, 51, 4024 DOI: 10.1039/C5CC00322A

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