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Issue 10, 2015
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A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

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Abstract

A strategy for T4 polynucleotide kinase activity detection was proposed based on a β-cyclodextrin polymer (polyβ-CD) and an exonuclease reaction. The fluorescence of pyrene enhanced by more than 10 times in the presence of polyβ-CD, and a simple detection of T4 PNK was achieved with a detection limit of 0.02 units per mL.

Graphical abstract: A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

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Publication details

The article was received on 11 Nov 2014, accepted on 10 Dec 2014 and first published on 10 Dec 2014


Article type: Communication
DOI: 10.1039/C4CC08991J
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 1815-1818
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    A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

    C. Song, X. Yang, K. Wang, Q. Wang, J. Liu, J. Huang, L. He, P. Liu, Z. Qing and W. Liu, Chem. Commun., 2015, 51, 1815
    DOI: 10.1039/C4CC08991J

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