Issue 73, 2014
From the journal:

Chemical Communications

Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease†

Lijuan Ji,a   Zhewei Cai,‡a   Yingdan Qian,a   Ping Wu,a   Hui Zhang*a  and  Chenxin Cai*a  

* Corresponding authors

a Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China
E-mail: zhangh@njnu.edu.cn, cxcai@njnu.edu.cn
Fax: +86 25 85891767
Tel: +86 25 85891780

Abstract

We report a sensitive and selective approach for the DNA methyltransferase (MTase) activity assay and MTase inhibitor screening by coupling the fluorescence quenching of graphene oxide with site-specific cleavage of a restriction endonuclease.

Graphical abstract: Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

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Article information

DOI
https://doi.org/10.1039/C4CC04428B
Article type
Communication
Submitted
11 Jun 2014
Accepted
23 Jul 2014
First published
24 Jul 2014

Chem. Commun., 2014,50, 10691-10694

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Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

L. Ji, Z. Cai, Y. Qian, P. Wu, H. Zhang and C. Cai, Chem. Commun., 2014, 50, 10691 DOI: 10.1039/C4CC04428B

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