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Issue 80, 2014
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A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells

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Abstract

For the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne–azide cycloaddition (copper free click) reaction (SPAAC). The ligation efficiency of SPAAC was improved by freeze–thaw cycles. The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF7, MDAMB453, Weri-RB1 and PC3.

Graphical abstract: A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells

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Publication details

The article was received on 23 Apr 2014, accepted on 27 May 2014 and first published on 19 Jun 2014


Article type: Communication
DOI: 10.1039/C4CC02996H
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Citation: Chem. Commun., 2014,50, 11810-11813
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    A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells

    N. Subramanian, J. B. Sreemanthula, B. Balaji, J. R. Kanwar, J. Biswas and S. Krishnakumar, Chem. Commun., 2014, 50, 11810
    DOI: 10.1039/C4CC02996H

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