Issue 80, 2014

A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells

Abstract

For the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne–azide cycloaddition (copper free click) reaction (SPAAC). The ligation efficiency of SPAAC was improved by freeze–thaw cycles. The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF7, MDAMB453, Weri-RB1 and PC3.

Graphical abstract: A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells

Supplementary files

Article information

Article type
Communication
Submitted
23 Apr 2014
Accepted
27 May 2014
First published
19 Jun 2014

Chem. Commun., 2014,50, 11810-11813

Author version available

A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells

N. Subramanian, J. B. Sreemanthula, B. Balaji, J. R. Kanwar, J. Biswas and S. Krishnakumar, Chem. Commun., 2014, 50, 11810 DOI: 10.1039/C4CC02996H

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