Issue 76, 2014
From the journal:

Chemical Communications

2′-Deoxyuridine conjugated with a reactive monobenzocyclooctyne as a DNA building block for copper-free click-type postsynthetic modification of DNA†

Claudia Stubinitzky,a   Gergely B. Cserép,b   Effi Bätzner,a   Péter Kele*b  and  Hans-Achim Wagenknecht*a  

* Corresponding authors

a Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany
E-mail: Wagenknecht@kit.edu
Fax: +49-721-608-44825
Tel: +49-721-608-47486

b “Lendület” Chemical Biology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok krt. 2, H-1117, Budapest, Hungary
E-mail: kele.peter@ttk.mta.hu

Abstract

The carboxymethylmonobenzocyclooctyne group attached to the 5-position of a 2′-deoxyuridine in DNA allows rapid and efficient copper-free postsynthetic modification as demonstrated with a far-red emitting fluorescent azide probe. Upon labeling strong fluorescence intensity enhancement is observed.

Graphical abstract: 2′-Deoxyuridine conjugated with a reactive monobenzocyclooctyne as a DNA building block for copper-free click-type postsynthetic modification of DNA

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Article information

DOI
https://doi.org/10.1039/C4CC02855D
Article type
Communication
Submitted
17 Apr 2014
Accepted
24 Jul 2014
First published
30 Jul 2014

Chem. Commun., 2014,50, 11218-11221

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2′-Deoxyuridine conjugated with a reactive monobenzocyclooctyne as a DNA building block for copper-free click-type postsynthetic modification of DNA

C. Stubinitzky, G. B. Cserép, E. Bätzner, P. Kele and H. Wagenknecht, Chem. Commun., 2014, 50, 11218 DOI: 10.1039/C4CC02855D

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