Issue 28, 2014
From the journal:

Chemical Communications

Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)†

Kuangwen Hsieh,‡a   Peter L. Mage,‡b   Andrew T. Csordas,c   Michael Eisensteinc  and  H. Tom Soh*abc  

* Corresponding authors

a Department of Mechanical Engineering, University of California, Santa Barbara, CA 93106, USA
E-mail: tsoh@engr.ucsb.edu
Fax: +1 805 8938651
Tel: +1 805 8937985

b Materials Department, University of California, Santa Barbara, CA 93106, USA

c Institute for Collaborative Biotechnologies, University of California, Santa Barbara, CA 93106, USA

Abstract

We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.

Graphical abstract: Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

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Article information

DOI
https://doi.org/10.1039/C4CC00540F
Article type
Communication
Submitted
21 Jan 2014
Accepted
17 Feb 2014
First published
28 Feb 2014

Chem. Commun., 2014,50, 3747-3749

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Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

K. Hsieh, P. L. Mage, A. T. Csordas, M. Eisenstein and H. Tom Soh, Chem. Commun., 2014, 50, 3747 DOI: 10.1039/C4CC00540F

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