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Issue 20, 2014
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Screening of α-glucosidase inhibitors from green tea extracts using immobilized enzymes affinity capture combined with UHPLC-QTOF MS analysis

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Abstract

An applicable affinity capture-based method was developed for screening of enzyme inhibitors from complex plant extracts directly. By elimination of false positives, three non-competitive α-glucosidase inhibitors were fished out from 400 μL green tea extract rapidly, using affinity capture of immobilized α-glucosidase combined with UHPLC-QTOF MS analysis.

Graphical abstract: Screening of α-glucosidase inhibitors from green tea extracts using immobilized enzymes affinity capture combined with UHPLC-QTOF MS analysis

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Publication details

The article was received on 21 Dec 2013, accepted on 09 Jan 2014 and first published on 16 Jan 2014


Article type: Communication
DOI: 10.1039/C3CC49681C
Citation: Chem. Commun., 2014,50, 2582-2584
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    Screening of α-glucosidase inhibitors from green tea extracts using immobilized enzymes affinity capture combined with UHPLC-QTOF MS analysis

    S. Deng, L. Xia and H. Xiao, Chem. Commun., 2014, 50, 2582
    DOI: 10.1039/C3CC49681C

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