Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling

Abstract

Limited spectral resolution in the proton dimension of NMR spectra is a severe problem in intrinsically disordered proteins. Here we show that homonuclear broadband proton decoupling of the direct and indirect dimensions of multi-dimensional NMR spectra significantly enhances their resolution.