Issue 5, 2014
From the journal:

Chemical Communications

Triplex-mediated analysis of cytosine methylation at CpA sites in DNA†

Marie W. Johannsen,‡a   Simon R. Gerrard,a   Tracy Melvinb  and  Tom Brown‡*a  

* Corresponding authors

a School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK
E-mail: tom.brown@chem.ox.ac.uk
Tel: +44 (0)1865 275413

b Optoelectronics Research Centre, University of Southampton, UK

Abstract

Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.

Graphical abstract: Triplex-mediated analysis of cytosine methylation at CpA sites in DNA

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Article information

DOI
https://doi.org/10.1039/C3CC45917A
Article type
Communication
Submitted
02 Aug 2013
Accepted
06 Nov 2013
First published
07 Nov 2013
This article is Open Access
Creative Commons BY license

Chem. Commun., 2014,50, 551-553

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Triplex-mediated analysis of cytosine methylation at CpA sites in DNA

M. W. Johannsen, S. R. Gerrard, T. Melvin and T. Brown, Chem. Commun., 2014, 50, 551 DOI: 10.1039/C3CC45917A

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