Issue 70, 2013
From the journal:

Chemical Communications

An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads†

Qiang Zhao*a  and  Jie Gaob  

* Corresponding authors

a Research Center for Environmental Science and Engineering, Shanxi University, Taiyuan 030006, China
E-mail: chemzhaoq@hotmail.com
Fax: +86-351-7011011
Tel: +86-351-7011011

b College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China

Abstract

Here we present a sensitive and specific assay for thrombin through the affinity capture of thrombin with mRNA display generated peptide aptamers on magnetic beads and the subsequent thrombin-involved enzymatic reaction. Thrombin at 50 fM can be detected.

Graphical abstract: An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads

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Article information

DOI
https://doi.org/10.1039/C3CC44400G
Article type
Communication
Submitted
11 Jun 2013
Accepted
02 Jul 2013
First published
02 Jul 2013

Chem. Commun., 2013,49, 7720-7722

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An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads

Q. Zhao and J. Gao, Chem. Commun., 2013, 49, 7720 DOI: 10.1039/C3CC44400G

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