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Issue 86, 2013
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5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase

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Abstract

Here, we first demonstrated that 5-MedCTP could be incorporated into the synthetic DNA template by the exonuclease deficient Klenow fragment with a much higher efficiency than dCTP and 5-hydroxymethyl-dCTP. Further, we first conducted a comparable study of primer extension reaction using templates containing deoxycytidine (dC) or 5-methyldeoxycytidine (5-mdC) for incorporating different triphosphates. Based on our findings, 5-methyldeoxycytidine could enhance the substrate activity of the Klenow fragment (exo-) and this feature could potentially be used in DNA methylation analysis.

Graphical abstract: 5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase

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Publication details

The article was received on 28 May 2013, accepted on 05 Sep 2013 and first published on 05 Sep 2013


Article type: Communication
DOI: 10.1039/C3CC44017F
Citation: Chem. Commun., 2013,49, 10085-10087
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    5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase

    T. Tian, S. Peng, H. Xiao, Y. Long, B. Fu, X. Zhang, S. Guo, S. Wang, X. Zhou, S. Liu and X. Zhou, Chem. Commun., 2013, 49, 10085
    DOI: 10.1039/C3CC44017F

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