Issue 78, 2013
From the journal:

Chemical Communications

Ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification†

Guohua Zhou,a   Xian Zhang,a   Xinghu Ji*a  and  Zhike He*a  

* Corresponding authors

a Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
E-mail: xhu@whu.edu.cn, zhkhe@whu.edu.cn
Tel: +86-27-6875-6557

Abstract

Terminal protection of small molecule linked DNA assays amplified by Exo III-aided DNA recycling is developed for the detection of streptavidin–biotin interaction, with a detection limit of 0.8 fM streptavidin in the “turn-on” state, indicating that the proposed method is an ultrasensitive platform for the detection of small molecule–protein interactions.

Graphical abstract: Ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3CC43621G
Article type
Communication
Submitted
14 May 2013
Accepted
23 Jul 2013
First published
23 Jul 2013

Chem. Commun., 2013,49, 8854-8856

Permissions

Request permissions

Ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification

G. Zhou, X. Zhang, X. Ji and Z. He, Chem. Commun., 2013, 49, 8854 DOI: 10.1039/C3CC43621G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements