Issue 87, 2013
From the journal:

Chemical Communications

Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein†

Seiji Sakamoto,*a   Mika Terauchi,a   Anna Hugo,a   Tanner Kim,a   Yasuyuki Arakia  and  Takehiko Wada*a  

* Corresponding authors

a Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan
E-mail: sakamoto@tagen.tohoku.ac.jp, hiko@tagen.tohoku.ac.jp

Abstract

A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is “covalently” cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.

Graphical abstract: Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

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Article information

DOI
https://doi.org/10.1039/C3CC43389G
Article type
Communication
Submitted
07 May 2013
Accepted
10 Sep 2013
First published
26 Sep 2013

Chem. Commun., 2013,49, 10323-10325

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Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

S. Sakamoto, M. Terauchi, A. Hugo, T. Kim, Y. Araki and T. Wada, Chem. Commun., 2013, 49, 10323 DOI: 10.1039/C3CC43389G

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