Issue 65, 2013
From the journal:

Chemical Communications

Directed co-evolution of an endoglucanase and a β-glucosidase in Escherichia coli by a novel high-throughput screening method†

Min Liu,a   Jiali Gu,a   Wenping Xiea  and  Hongwei Yu*a  

* Corresponding authors

a Institute of Bioengineering and State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, P.R. China
E-mail: yuhongwei@zju.edu.cn
Fax: +86-571-8795 1873
Tel: +86-571-8795 1873

Abstract

A novel high-throughput screening method for directed co-evolution of endoglucanase and β-glucosidase was constructed based on the synergy of the two enzymes using a bicistronic operon. To our knowledge, this is the first report of directed co-evolution of two cellulosic enzymes using an insoluble screening substrate via their synergistic reactions.

Graphical abstract: Directed co-evolution of an endoglucanase and a β-glucosidase in Escherichia coli by a novel high-throughput screening method

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Article information

DOI
https://doi.org/10.1039/C3CC42485E
Article type
Communication
Submitted
06 Apr 2013
Accepted
21 Jun 2013
First published
21 Jun 2013

Chem. Commun., 2013,49, 7219-7221

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Directed co-evolution of an endoglucanase and a β-glucosidase in Escherichia coli by a novel high-throughput screening method

M. Liu, J. Gu, W. Xie and H. Yu, Chem. Commun., 2013, 49, 7219 DOI: 10.1039/C3CC42485E

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