Issue 51, 2013

Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format

Abstract

The electrochemical detection of dehydrogenase activity in crude cell lysates is performed simultaneously using 96 carbon electrodes modified with electrografted phenazines. The method is applied to the screening of a library of formate dehydrogenase mutants obtained by directed evolution.

Graphical abstract: Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format

Supplementary files

Article information

Article type
Communication
Submitted
21 Mar 2013
Accepted
30 Apr 2013
First published
01 May 2013

Chem. Commun., 2013,49, 5781-5783

Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format

S. Abdellaoui, M. Bekhouche, A. Noiriel, R. Henkens, C. Bonaventura, L. J. Blum and B. Doumèche, Chem. Commun., 2013, 49, 5781 DOI: 10.1039/C3CC42065E

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