Issue 9, 2013

Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing

Abstract

Functional microfluidic free-flow electrophoresis chips with integrated fluorescent pH sensors are presented. Polyethylene glycol based structures were fabricated that allowed for integration of both functions on a single microchip. Microchips were applied in free-flow isoelectric focusing of model compounds and proteins with on-line monitoring of pH during microscale electrophoretic separation.

Graphical abstract: Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing

Supplementary files

Article information

Article type
Communication
Submitted
08 Nov 2012
Accepted
10 Dec 2012
First published
12 Dec 2012

Chem. Commun., 2013,49, 904-906

Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing

S. Jezierski, D. Belder and S. Nagl, Chem. Commun., 2013, 49, 904 DOI: 10.1039/C2CC38093E

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